Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Tris Buffered Saline, TBS (20X) - 4L
The Tris Buffered Saline (TBS) is an isotonic solution frequently used in biological research due to its non-toxic nature. The formulation of TBS includes Tris (tris(hydroxymethyl)aminomethane), which serves as a buffering agent, and Sodium Chloride (NaCl) salt. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 20X concentration, and is useful for washing in Western Blotting.
Boston Bioproducts Inc MES Buffer (0.5 M, pH 5.5) - 250ml
MES Buffer [2- (N-morpholino) ethanesulfonic acid] is a zwitterionic Good's buffer used in biological and biochemical research. It maintains a stable pH within the range of 5.5 to 7.0 and is highly soluble in water. MES is commonly used as a running buffer for bis-tris gels, chromatography, and in protein purification. It is valuable for enzymatic reactions, and acts as a non-coordinating buffer in metal ion chemistry. It has minimal UV absorbance and is stable in various applications.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 8.5) - 1L
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Polysciences, Inc. 1M Glycine-HCl Buffer, pH 2.7±0.1, 10X Concentrate 500 ML
This item has a minimum qty of 2 per supplier requirements.
1M GLYCINE-HCL BUFFER 500 ML
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 6.5) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid anhydrous, crystalline, BioReagent, suitable for cell culture | 60-00-4 | MFCD00003541 | 500g
Ethylenediaminetetraacetic acid anhydrous, crystalline, BioReagent, suitable for cell culture | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 500g
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline powder, pH 7.4, for preparing 5 L solutions | MFCD00131855 | 10/pk
Phosphate buffered saline powder, pH 7.4, for preparing 5 L solutions | | MFCD00131855 | 10/pk
Boston Bioproducts Inc EDTA (0.5 M, pH 7.4, pH Adjusted with KOH) - 250ml
EDTA [Ethylenediaminetetraacetic acid] solution is commonly used in various molecular biology and protein biochemistry applications that require the chelation of divalent metal cations, such as calcium (Ca2+) and magnesium (Mg2+). EDTA is used extensively as an anticoagulant in blood samples as it chelates the calcium present, preventing coagulation and preserving blood cell morphology. It is also used in cell lysis buffers. This solution is adjusted with KOH.
Teknova 200mM Tris, pH 7.0. 1mL, Sterile. 50 Pack
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Sigma Aldrich Fine Chemicals Biosciences Dulbecco's Phosphate Buffered Saline Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 100ML
Dulbecco's Phosphate Buffered Saline Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 100ML
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline BioPerformance Certified, pH 7.4 | MFCD00131855 | 10PAK
Phosphate buffered saline BioPerformance Certified, pH 7.4 | MFCD00131855 | 10PAK
Discovery Life Sciences .5M POTASSSIUM PHOS BUFFER 7.4
Potassium Phosphate Buffer, P450 Enzyme Buffer, 0.5M