Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Worthington Biochemical Corporation EARK=LE'S BUFFER VIAL
Earle's balanced salt solution (EBSS) as supplied in the Papain Dissociation System.
Teknova 20X PBS with 1% Tween-20, pH 7.3 (1000mL, Sterile)
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Boston Bioproducts Inc Tris Buffered Saline, TBS (20X, for Western Blot Washing) - 1L
The Tris Buffered Saline (TBS) is an isotonic non-toxic buffer solution used commonly in biochemical applications. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 20X concentration, and is useful for washing in Western Blotting.
IBI SCIENTIFIC 3P 10X TBE Buffer, 1 liter: Superior Buffering Capacity for Electrophoresis Applications
TBE (Tris / Boric Acid / EDTA) buffer is a high-performance solution designed to offer superior buffering capacity compared to TAE. With its enhanced ability to maintain pH stability over extended electrophoresis runs, TBE is the preferred choice for DNA sequencing, gel electrophoresis, and other applications requiring high-resolution separation. This buffer eliminates the need for recirculation in most electrophoresis protocols, making it more efficient and reliable for laboratories conducting extended gel runs.
Sigma Aldrich Fine Chemicals Biosciences HANKS BALANCED SALT SOLUTION
NC3660344 HANKS BALANCED SALT SOLUTION
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 6.5) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.5) - 250ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging.
Sciencell Research Laboratories DULBECCO'S PHOSPHATE-BUFFERED
Dulbecco's Phosphate-Buffered Saline 500 ml
Bioworld BSA (1%) IN PBS, DNASE AND RNA
BSA (1%) in PBS, DNase and RNase Free0.79% Sodium Chloride, 0.02% Potassium Chloride, 0.06% Sodium Phosphate, 0.02% Potassium Phosphate, 1.0% BSA, DNAse/RNAse-Free