Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![eMolecules 39946-25-3 | 2-(N-MORPHOLINO)ETHANESULFONIC ACID POTASSIUM SALT | AstaTech | MFCD00069752 | 233.320 | C6H12KNO4S | 95.000 | [K+].[O-]S(=O)(=O)CCN1CCOCC1 | 100g | 696752925](https://assets.fishersci.com/TFS-Assets/CCG/product-images/NC3454793.JPG-250.jpg)
eMolecules 39946-25-3 | 2-(N-MORPHOLINO)ETHANESULFONIC ACID POTASSIUM SALT | AstaTech | MFCD00069752 | 233.320 | C6H12KNO4S | 95.000 | [K+].[O-]S(=O)(=O)CCN1CCOCC1 | 100g | 696752925
2-(N-MORPHOLINO)ETHANESULFONIC ACID POTASSIUM SALT | AstaTech | 39946-25-3 | MFCD00069752 | 233.320 | C6H12KNO4S | 95.000 | [K+].[O-]S(=O)(=O)CCN1CCOCC1 | 100g | 696752925
Fd Neurotechnologies Inc PHOSPHATE-BUFFERED 4% PARAFORM
This 1000ml filtered and ready-to-use tissue fixative is freshly made from high purity paraformaldehyde powder. This solution may be stored at room temperature for at least one year without change of the original quality. The standard solution contains 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.4). Other concentrations can also be provided upon request.
Boston Bioproducts Inc MES Buffer (0.5 M, pH 6.5) - 250ml
MES Buffer [2- (N-morpholino) ethanesulfonic acid] is a zwitterionic Good's buffer used in biological and biochemical research. It maintains a stable pH within the range of 5.5 to 7.0 and is highly soluble in water. MES is commonly used as a running buffer for bis-tris gels, chromatography, and in protein purification. It is valuable for enzymatic reactions, and acts as a non-coordinating buffer in metal ion chemistry. It has minimal UV absorbance and is stable in various applications.
Sigma Aldrich Fine Chemicals Biosciences Phosphate Buffered Saline | 1 L
Phosphate buffered saline | Purity: 100% | Mol Wt: N/A | CAS No: N/A | MFCD00011392 | 1LPhosphate buffered saline is an isotonic balanced salt solution used for washing cells and tissues while maintaining physiological pH and osmolarity. It contains sodium chloride, sodium phosphate dibasic, potassium chloride and potassium phosphate monobasic formulated to mimic salt composition, pH and osmolarity of the human body. Lack of calcium and magnesium prevents clotting with blood cells. PBS maintains cell viability and is useful for diluting cells for counting and antibodies for immunofluorescence microscopy. PBS is supplied sterile-filtered without preservatives or endotoxins.
Fd Neurotechnologies Inc PHOSPH-BUF .04PARAFORM W/ PA
Phosphate-Buffered 0.04 Paraformaldehyde with Picric Acid
Sigma Aldrich Fine Chemicals Biosciences Tris Buffered Saline, MFCD00132476, 1 L
Synonym: TBS, 10 ×, solution. Tris Buffered Saline has been used as washing buffer for electrodes. It was used to wash microtiter plates for western blot experiments and screening methods.
VWR INTERNATIONAL LLC Phosphate Buffered Saline (PBS) 20X, Ultra Pure Grade
1X Solution Composition:135 mM NaCl, 2.7 mM KCl,11 mM Phosphate Buffer. Does not contain magnesium or calcium.
HORIZON DISCOVERY BIOSCIENCES LIMITED 10MM TRIS-HCL BUFFER PH 7.4
NC2372517 10MM TRIS-HCL BUFFER PH 7.4
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.5) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 9.5) - 1L
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.